Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism of acetaminophen and Evaluating the Role of Susceptibility Inducing Cofactors and of Acetaminophen in the Etiology of Autism Spectrum Disorder Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.