Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Full article: Metabolic phenotyping applied to pre clinical and clinical studies of acetaminophen metabolism and hepatotoxicity Metabolism of acetaminophen (paracetamol) in plantstwo independent pathways result in the formation of a glutathione and a glucose conjugate Environmental Science and Pollution Research Springer Nature Link