Should I Take Glutathione On An Empty Stomach Or With Food? Plixlife Best Time to Take Glutathione: Morning or Night? Healeo Nutrition Glutathione is such a critical soldier in our line of defense that our bodies make it themselves. We even have mechanisms that recycle and reuse it. But what happens when our toxic Wondering about the best time to take your vitamins? Some are better taken in the morning, while others with a meal. Consistency is key, so take them at the same time daily.