Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen Glutathione Disodium Salt CAS 64889 81 2 (free acid) SCBT Santa Cruz Biotechnology Clinical toxicity of Acetaminophen Here's how to make Tylenol work way better Dose it correctly: 1,000 mg at a time, spaced every 8 hours. Smaller doses don't control pain as well. Take it