Acetaminophen Depletes Glutathione Essential Formulas Sex differences in glutathione metabolism and acetaminophen toxicity View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.