The molecular mechanisms of acetaminophen induced hepatotoxicity and its potential therapeutic targets Guangwen Luo, Lili Huang, Zhaowei Zhang, 2023 TYLENOL [Acetaminophen] metabolism A lot of GLUTATHIONE is needed to deal with the Oxidative Stress generated by the CYP 450 metabolism of Tylenol. When people go to the ER for Tylenol Overdose Solved The stepwise mechanism below shows how glutathione Chegg.com Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.