Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns: STAR Protocols Glutathione Sepharose 4B GST tagged protein purification resin Cytiva Cytiva Glutathione Sepharose 4B Genesee Scientific Unlocking the secrets of SARS CoV 2 nsp3 by combining experiments with AlphaFold2 domain prediction Life Science Alliance
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