Glutathione Sepharose 4 Fast Flow GST tagged protein purification resin Cytiva Sigma Aldrich Activated ThiolSepharose 4B, lyophilized Powder 1 G Cytiva Glutathione Sepharose 4B Genesee Scientific Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns: STAR Protocols
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