Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Acetaminophen Depletes Glutathione Essential Formulas Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.