Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione Deficiency Symptoms: Is Poor Sleep Just the Beginning? Cardiology & Neurology Specialists located in Queens, Forest Hills and Brighton Beach, Brooklyn, NY Advanced Medical Care Deficient Glutathione in the Pathophysiology of Mycotoxin Related Illness Since Tylenol is such a hot topic right now, did you know it depletes glutathione levels? When your body processes Tylenol in the liver, one of the pathways creates a toxic byproduct. To neutralize