The Metabolism of Acetaminophen and the Synthesis of Glutathione Download Scientific Diagram Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. A Possible Antidote for Tylenol (Paracetamol) Overdose American Council on Science and Health Tylenol and Glutathione: A Closer Look At Their Relationship